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Microfluidic droplet based enzyme variant screening: Towards improved enzymes for industrial applications
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).ORCID iD: 0000-0001-5232-0805
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
2011 (English)In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2011, 179-181 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present a microdroplet based assay for selection of efficient variants of bacterially produced amylase enzyme to improve these enzymes for industrial applications. Fluorescent analysis of α-amylase in droplets at relevant concentrations demonstrates the discrimination of wild type α-amylase at stressed and non-stressed conditions. Dielectrophoretic sorting enables enrichment of target droplets from 48% to 98.1%. Finally the viability and proliferation of Bacillus Subtilis in droplets is demonstrated. This demonstrates an enzyme analysis and screening assay in the microfluidic droplets format for selection of an industrially relevant enzyme and a basis for further enzyme selections where fluorogenic substrates are available.

Place, publisher, year, edition, pages
2011. 179-181 p.
Keyword [en]
Cells, Droplet microfluidics, Enzymes, High throughput, Bacillus Subtilis, Dielectrophoretic, Fluorescent analysis, Fluorogenic substrate, Microfluidic droplets, Screening assays, Drops, Industrial applications, Microfluidics, Amylases
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148749Scopus ID: 2-s2.0-84883795480ISBN: 9781618395955 (print)OAI: oai:DiVA.org:kth-148749DiVA: diva2:745284
Conference
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
Note

QC 20140910

Available from: 2014-09-10 Created: 2014-08-11 Last updated: 2014-09-10Bibliographically approved

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Jönsson, Håkan

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Output format
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