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Bacteria isolation from whole blood for sepsis diagnostics
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0001-5199-0663
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0001-8531-5607
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
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2011 (English)In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2011, 518-520 p.Conference paper, Published paper (Refereed)
Abstract [en]

Rapid and reliable detection of bloodstream infections would gain a lot from improved and straightforward isolation of highly purified bacteria from whole blood. Here, we report a microfluidics-based sample preparation strategy to continuously isolate microorganisms from whole blood for downstream analysis. The continuous-flow method takes advantage of the fact that bacteria cells have rigid cell wall enables selective and complete blood cell lysis while ~ 100% of bacteria are readily recovered. The method as a sample preparation unit offers opportunities to develop molecular based POC for sepsis diagnostics.

Place, publisher, year, edition, pages
2011. 518-520 p.
Keyword [en]
Bacteria isolation, Infectious disease, Selective cell lysis, Sepsis, Bacteria cell, Cell lysis, Continuous-flow, Reliable detection, Sample preparation, Blood, Bacteria
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148697Scopus ID: 2-s2.0-84883767207ISBN: 9781618395955 (print)OAI: oai:DiVA.org:kth-148697DiVA: diva2:746345
Conference
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
Note

QC 20140912

Available from: 2014-09-12 Created: 2014-08-11 Last updated: 2014-09-12Bibliographically approved

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Hansson, JonasBrismar, Hjalmar

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