Controlling acoustic streaming in a multi-well microplate for improving live cell assays
2011 (English)In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2011, 1612-1614 p.Conference paper (Refereed)
Acoustic streaming in a multi-well microplate is investigated using two different ultrasonic actuation frequency-schemes: Single-frequency and frequency-modulation. The streaming is tracked by the use of 1 μm fluorescent polymer beads and micro-particle image velocimetry. The suspension also contained human B cells for studying the acoustic trapping and aggregation performance simultaneously with the acoustic streaming. Our results show a significant difference in the acoustic streaming between the two ultrasonic actuation schemes. A rotational fluid flow speed decreased a factor of 30 when frequency-modulation was applied compared to single-frequency actuation without apparently interfering with the acoustic cell trapping function.
Place, publisher, year, edition, pages
2011. 1612-1614 p.
Acoustic streaming, Microplate, Mixing, Multi-well, Particle image velocimetry, Ultrasound, Cell trapping, Fluorescent polymers, Live cell, Micro particle image velocimetry, Microplates, Multi wells, Particle image velocimetries, Single-frequency, Flow visualization, Modulation, Ultrasonics, Velocimeters, Velocity measurement
IdentifiersURN: urn:nbn:se:kth:diva-148694ScopusID: 2-s2.0-84883821752ISBN: 9781618395955OAI: oai:DiVA.org:kth-148694DiVA: diva2:746358
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
QC 201409122014-09-122014-08-112014-09-12Bibliographically approved