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Controlling acoustic streaming in a multi-well microplate for improving live cell assays
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-7023-4772
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-3996-9279
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0001-5178-7593
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2011 (English)In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2011, 1612-1614 p.Conference paper, Published paper (Refereed)
Abstract [en]

Acoustic streaming in a multi-well microplate is investigated using two different ultrasonic actuation frequency-schemes: Single-frequency and frequency-modulation. The streaming is tracked by the use of 1 μm fluorescent polymer beads and micro-particle image velocimetry. The suspension also contained human B cells for studying the acoustic trapping and aggregation performance simultaneously with the acoustic streaming. Our results show a significant difference in the acoustic streaming between the two ultrasonic actuation schemes. A rotational fluid flow speed decreased a factor of 30 when frequency-modulation was applied compared to single-frequency actuation without apparently interfering with the acoustic cell trapping function.

Place, publisher, year, edition, pages
2011. 1612-1614 p.
Keyword [en]
Acoustic streaming, Microplate, Mixing, Multi-well, Particle image velocimetry, Ultrasound, Cell trapping, Fluorescent polymers, Live cell, Micro particle image velocimetry, Microplates, Multi wells, Particle image velocimetries, Single-frequency, Flow visualization, Modulation, Ultrasonics, Velocimeters, Velocity measurement
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148694Scopus ID: 2-s2.0-84883821752ISBN: 9781618395955 (print)OAI: oai:DiVA.org:kth-148694DiVA: diva2:746358
Conference
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
Note

QC 20140912

Available from: 2014-09-12 Created: 2014-08-11 Last updated: 2014-09-12Bibliographically approved

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Ohlin, MathiasÖnfelt, Björn

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