Rapid melting curve analysis on monolayered beads for high-throughput genotyping of single-nucleotide polymorphisms
2006 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 78, no 7, 2220-2225 p.Article in journal (Refereed) Published
This report describes a rapid solid-phase melting curve analysis method for single-nucleotide polymorphism (SNP) genotyping. The melting curve analysis is based on dynamic allele-specific hybridization (DASH). The DNA duplexes are conjugated on beads that are immobilized on the surface of a microheater chip with integrated heaters and temperature sensors. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. The method is based on random bead immobilization by microcontact printing. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Analyses that took more than 15 min could be performed in less that 1 min, enabling ultrarapid SNP analysis. In addition, an array version of the chip was implemented enabling the preparation of an array of bead arrays for high-throughput and rapid SNP genotyping.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2006. Vol. 78, no 7, 2220-2225 p.
allele-specific hybridization, real-time, dna, system, arrays
IdentifiersURN: urn:nbn:se:kth:diva-5012DOI: 10.1021/ac051771uISI: 000236686600022PubMedID: 16579600ScopusID: 2-s2.0-33645660631OAI: oai:DiVA.org:kth-5012DiVA: diva2:7466
Uppdaterad från manuskript till artikel: 20101008. QC 201010082005-04-012005-04-012016-07-22Bibliographically approved