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Development of microfluidic aqueous two-phase system for continuous partitioning of E. coli strains
KTH, School of Biotechnology (BIO), Bioprocess Technology (closed 20130101). KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0003-3618-9944
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0001-5199-0663
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0001-8531-5607
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
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2011 (English)In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2011, 1329-1331 p.Conference paper, Published paper (Refereed)
Abstract [en]

The interaction of bacterial cells with surrounding environment depends on its surface characteristics such as hydrophobicity, hydrophilicity balance and net charge. In this paper, aqueous two-phase system partitioning of Escherichia coli strains based on their difference in surface properties is introduced in a microfluidic system. While aqueous two-phase system is widely use to separate biomolecules on macroscale, the method has not been adapted in microfluidic system. The bacterial cells are partitioned based on their affinity for streams formed by aqueous polymers polyethylene glycol (PEG) and dextran (Dex). Partitioning efficiency of two Escherichia coli strains is currently being optimized.

Place, publisher, year, edition, pages
2011. 1329-1331 p.
Keyword [en]
Aqueous two phase system, Continuous partitioning, Dex, Escherichia coli, Microfluidics, PEG, Bacterial cells, Micro fluidic system, Polyethylene glycol (PEG), Surface characteristics, Surrounding environment, Fluidic devices, Polyethylene glycols, Solvent extraction
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-148687Scopus ID: 2-s2.0-84883748231ISBN: 9781618395955 (print)OAI: oai:DiVA.org:kth-148687DiVA: diva2:746917
Conference
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, 2 October 2011 through 6 October 2011, Seattle, WA
Note

QC 20140915

Available from: 2014-09-15 Created: 2014-08-11 Last updated: 2014-09-15Bibliographically approved

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Periyannan Rajeswari, Prem KumarHansson, Jonas

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Periyannan Rajeswari, Prem KumarRamachandraiah, HarishaHansson, JonasArdabili, SaharVeide, AndresRussom, Aman
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Bioprocess Technology (closed 20130101)Cell Physics
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