Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
3D image deconvolution in light microscopy: theory and practice
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
2000 (English)Report (Other academic)
Abstract [en]

The objective of this study is to investigate the use of deconvolution for improving the three-dimensional resolution in both confocal and wide-field light microscopy. We start by assessing the theoretical limits of the deconvolution by defining and calculating a characteristic function which describes the imaging-deconvolution process. We continue by suggesting a variant of inverse filtering for deconvolving real noisy data and describe the application of the method to a confocal recording of a biological preparation. The result is comparable with that obtainable by Wiener filtering but we can avoid the problem of finding a best value of the parameter. We also describe a way of determining the optical transfer function of the microscope.

Place, publisher, year, edition, pages
KTH Royal Institute of Technology, 2000. , 18 p.
Keyword [en]
Light microscopy, 3D imaging, deconvolution, characteristic function
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-150954OAI: oai:DiVA.org:kth-150954DiVA: diva2:747276
Note

QC 20140918

Available from: 2014-09-16 Created: 2014-09-11 Last updated: 2014-09-18Bibliographically approved

Open Access in DiVA

restore.pdf(600 kB)308 downloads
File information
File name FULLTEXT01.pdfFile size 600 kBChecksum SHA-512
d5c67a9cfe61e3ce9e5879ea7ab3e4d157e7ea7df41fafc734e230e2210a815f875835ffc97eb470422069a2cf8be2e7e617591c93a82461df8dad0698151dc0
Type fulltextMimetype application/pdf
fulltext(600 kB)180 downloads
File information
File name FULLTEXT02.pdfFile size 600 kBChecksum SHA-512
d5c67a9cfe61e3ce9e5879ea7ab3e4d157e7ea7df41fafc734e230e2210a815f875835ffc97eb470422069a2cf8be2e7e617591c93a82461df8dad0698151dc0
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Philip, JohanCarlsson, Kjell
By organisation
Applied PhysicsBiomedical and X-ray Physics
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 488 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 57 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf