Distinguishing xyloglucanase activity in endo-β(1 → 4)glucanases
2012 (English)In: Methods in Enzymology, ISSN 0076-6879, Vol. 510, 97-120 p.Article in journal (Refereed) Published
The ability of beta-glucanases to cleave xyloglucans, a family of highly decorated beta-glucans ubiquitous in plant biomass, has traditionally been overlooked in functional biochemical studies. An emerging body of data indicates, however, that a spectrum of xyloglucan specificity resides in diverse glycoside hydrolases from a range of carbohydrate-active enzyme families including classic "cellulase" families. This chapter outlines a series of enzyme kinetic and product analysis methods to establish degrees of xyloglucan specificity and modes of action of glycosidases emerging from enzyme discovery projects.
Place, publisher, year, edition, pages
2012. Vol. 510, 97-120 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-152114DOI: 10.1016/B978-0-12-415931-0.00006-9ISI: 000308194700006PubMedID: 22608723ScopusID: 2-s2.0-84861123861ISBN: 978-0-12-415931-0OAI: oai:DiVA.org:kth-152114DiVA: diva2:749577
QC 201409242014-09-242014-09-232014-09-24Bibliographically approved