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Bacterial Nanoscale Cultures for Phenotypic Multiplexed Antibiotic Susceptibility Testing
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
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2014 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 52, no 9, 3310-3317 p.Article in journal (Refereed) Published
Abstract [en]

An optimal antimicrobial drug regimen is the key to successful clinical outcomes of bacterial infections. To direct the choice of antibiotic, access to fast and precise antibiotic susceptibility profiling of the infecting bacteria is critical. We have developed a high-throughput nanowell antibiotic susceptibility testing (AST) device for direct, multiplexed analysis. By processing in real time the optical recordings of nanoscale cultures of reference and clinical uropathogenic Escherichia coli strains with a mathematical algorithm, the time point when growth shifts from lag phase to early logarithmic phase (T-lag) was identified for each of the several hundreds of cultures tested. Based on T-lag, the MIC could be defined within 4 h. Heatmap presentation of data from this high-throughput analysis allowed multiple resistance patterns to be differentiated at a glance. With a possibility to enhance multiplexing capacity, this device serves as a high-throughput diagnostic tool that rapidly aids clinicians in prescribing the optimal antibiotic therapy.

Place, publisher, year, edition, pages
2014. Vol. 52, no 9, 3310-3317 p.
National Category
Microbiology in the medical area
URN: urn:nbn:se:kth:diva-153263DOI: 10.1128/JCM.01161-14ISI: 000341359300027ScopusID: 2-s2.0-84906859318OAI: diva2:753857

QC 20141009

Available from: 2014-10-09 Created: 2014-10-03 Last updated: 2014-10-09Bibliographically approved

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Weibull, EmilieAndersson-Svahn, Helene
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