In situ forming spruce xylan-based hydrogel for cell immobilization
2014 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 102, 862-868 p.Article in journal (Refereed) Published
An in situ forming spruce xylan-based hydrogel was synthesized in two steps with the intended use of cell encapsulation and in vivo delivery. First, bioconjugate was obtained through the reaction of glucuronic acid groups from xylan backbone with tyramine (TA). After that, the gelation process was enabled by enzymatic crosslinking of the phenol-containing TA-xylan conjugate. Exhibiting an exponential increase in the storage modulus, a 3D gel network was formed in about 20s. The designed gel showed extensive swelling and retained its mechanical integrity for more than two months. Mesenchymal stem cells were encapsulated in the hydrogel and cultured for one week. The cells retained their adipogenic differentiation capacity inside the gel, as verified by lipid accumulation. From these facts, we conclude that spruce xylan is a promising precursor for in situ forming hydrogels and should be evaluated further for tissue engineering purposes.
Place, publisher, year, edition, pages
2014. Vol. 102, 862-868 p.
Hydrogel, Xylan, Conjugate, Enzymatic crosslinking, Cell immobilization
IdentifiersURN: urn:nbn:se:kth:diva-153694DOI: 10.1016/j.carbpol.2013.10.077ISI: 000331779600111ScopusID: 2-s2.0-84893776032OAI: oai:DiVA.org:kth-153694DiVA: diva2:755359
FunderKnut and Alice Wallenberg Foundation
QC 201410142014-10-142014-10-072014-10-14Bibliographically approved