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Role of (1,3)(1,4)-beta-Glucan in Cell Walls: Interaction with Cellulose
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2014 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 15, no 5, 1727-1736 p.Article in journal (Refereed) Published
Abstract [en]

(1,3)(1,4)-beta-D-Glucan (mixed-linkage glucan or MLG), a characteristic hemicellulose in primary cell walls of grasses, was investigated to determine both its role in cell walls and its interaction with cellulose and other cell wall polysaccharides in vitro. Binding isotherms showed that MLG adsorption onto microcrystalline cellulose is slow, irreversible, and temperature-dependent. Measurements using quartz crystal microbalance with dissipation monitoring showed that MLG adsorbed irreversibly onto amorphous regenerated cellulose, forming a thick hydrogel. Oligosaccharide profiling using endo-(1,3)(1,4)-beta-glucanase indicated that there was no difference in the frequency and distribution of (1,3) and (1,4) links in bound and unbound MLG. The binding of MLG to cellulose was reduced if the cellulose samples were first treated with certain cell wall polysaccharides, such as xyloglucan and glucuronoarabinoxylan. The tethering function of MLG in cell walls was tested by applying endo-(1,3)(1,4)-beta-glucanase to wall samples in a constant force extensometer. Cell wall extension was not induced, which indicates that enzyme-accessible MLG does not tether cellulose fibrils into a load-bearing network.

Place, publisher, year, edition, pages
2014. Vol. 15, no 5, 1727-1736 p.
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Polymer Chemistry
URN: urn:nbn:se:kth:diva-153686DOI: 10.1021/bm5001247ISI: 000335939800019ScopusID: 2-s2.0-84900452471OAI: diva2:755389
Knut and Alice Wallenberg Foundation

QC 20141014

Available from: 2014-10-14 Created: 2014-10-07 Last updated: 2014-10-14Bibliographically approved

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Gatenholm, Paul
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Polymer Chemistry

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