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The impact of horizontal offset of the cornea during corneal specular microscopy
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
2008 (English)In: Progr. Biomed. Opt. Imaging Proc. SPIE, 2008Conference paper (Refereed)
Abstract [en]

We are developing automated morphometric analysis of the corneal endothelium. Here, the general impact of horizontal offset of the cornea on morphometry was examined. Errors due to perspective during imaging with a Clinical Specular Microscope (CSM) were analyzed considering semi automated analysis software and fully automated Fourier analysis software. Methods: A mathematical model of the cornea was created. Trigonometry was applied to find the relationship between the horizontal offset of the cornea relative to the microscope objective, and the consecutive errors from perspective changes in the image. An experimental setup was created using a cornea made of polymethyl methacrylate (PMMA). The posterior surface of the PMMA cornea was horizontally marked. The PMMA cornea was placed in a holder. Difference in refractive index between real endothelium and aqueous humor was emulated using high refractive index liquid. Images with varying horizontal offset on the PMMA corneal posterior surface, along with their relative offset coordinates were captured, using CSM. Results: Experiments using controlled offset of the cornea in relation to its center estimated that analyzable images can be acquired within an interval of 1.26 mm, using central cornea sampling CSM. Because of refractive indices along with light scattering differences between the corneal tissue and PMMA , the 1.26 mm interval should be considered a first estimate for feasible CSM images. The effect of corneal endothelial offset during imaging with CSM or fully automated Fourier analysis should be considered.

Place, publisher, year, edition, pages
, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, ISSN 1605-7422 ; 6844
Keyword [en]
Cornea, Endothelium, Fourier, Microscopy, Morphometrics, Offset, Computer software, Endothelial cells, Fourier analysis, Light scattering, Mathematical models, Polymethyl methacrylates, Corneal specular microscopy, Biological organs
National Category
Radiology, Nuclear Medicine and Medical Imaging
URN: urn:nbn:se:kth:diva-154458DOI: 10.1117/12.779573ISI: 000254982000020ScopusID: 2-s2.0-43949109291ISBN: 9780819470195OAI: diva2:758313
Ophthalmic Technologies XVIII, 19-21 January 2008, San Jose, CA, USA

QC 20141027

Available from: 2014-10-27 Created: 2014-10-20 Last updated: 2014-10-27Bibliographically approved

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