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Single molecule detection at surfaces: Dual-color fluorescence fluctuation spectroscopy with total internal reflection excitation
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics. École Polytechnique Fédé rale de Lausanne, Switzerland .
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2007 (English)In: New Approaches in Biomedical Spectroscopy, American Chemical Society (ACS), 2007, 259-279 p.Conference paper, Published paper (Refereed)
Abstract [en]

Fluorescence fluctuation spectroscopy based on an evanescent field excitation scheme leads to an alternative concept for single molecule detection with interesting experimental features. This concept is described and analyzed regarding the total fluorescence process including excitation, fluorescence emission and collection close to a dielectric interface. The realized experimental scheme showed superior performance of this concept with substantially enhanced molecular brightness when compared to the classical confocal setup based on water immersion objectives. Selected applications in life sciences including dual-color surface fluorescence correlation spectroscopy, enzyme kinetics and receptor-ligand binding are underlining the interest of this experimental approach.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2007. 259-279 p.
Series
ACS Symposium Series, ISSN 0097-6156 ; 963
Keyword [en]
High Count-Rate, Interfaces, Scattering, Diffusion, Dynamics, Binding, Dipole, Light
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-155553ISI: 000269122900017Scopus ID: 2-s2.0-36749060776ISBN: 0841274371 (print)ISBN: 978-084127437-2 OAI: oai:DiVA.org:kth-155553DiVA: diva2:762510
Conference
Symposium on Approaches in Biomedical Spectroscopy, Honolulu, HI, 2005
Note

QC 20141114

Available from: 2014-11-12 Created: 2014-11-07 Last updated: 2014-11-12Bibliographically approved

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