Safety study of an antimicrobial peptide lactocin 160, produced by the vaginal Lactobacillus rhamnosus
2007 (English)In: Infectious diseases in obstetrics and gynecology, ISSN 1064-7449, E-ISSN 1098-0997, Vol. 2007, 78248- p.Article in journal (Refereed) Published
Objective. To evaluate the safety of the antimicrobial peptide, lactocin 160. Methods. Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal) was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50). Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspension. Susceptibility of lactobacilli to lactocin160 was also studied. Rabbit vaginal irritation (RVI) model was used for an in vivo safety evaluation. Results. The ET-50 value was 17.5 hours for lactocin 160 (4.9 hours for nonoxynol 9, N9). Hemolytic activity of lactocin 160 was 8.2% (N9 caused total hemolysis). Lactobacilli resisted to high concentrations of peptide preparation. The RVI model revealed slight vaginal irritation. An average irritation index grade was evaluated as "none." Conclusions. Lactocin 160 showed minimal irritation and has a good potential for intravaginal application.
Place, publisher, year, edition, pages
2007. Vol. 2007, 78248- p.
animal experiment, animal model, antibiotic resistance, antimicrobial activity, article, cell suspension, drug delivery system, drug safety, drug screening, erythrocyte, exposure variable, female, hemolysis, intravaginal application, Lactobacillus rhamnosus, nonhuman, priority journal, rabbit, tissue characterization, tissue viability, toxicity, vagina flora, vaginal discomfort, vaginal irritation, vaginitis, animal, chemistry, comparative study, cytochemistry, drug effect, human, intravaginal drug administration, microbiological examination, pathology, vagina
Other Medical Sciences
IdentifiersURN: urn:nbn:se:kth:diva-155269DOI: 10.1155/2007/78248PubMedID: 18273406ScopusID: 2-s2.0-38749099818OAI: oai:DiVA.org:kth-155269DiVA: diva2:765911
QC 201411252014-11-252014-11-042014-11-25Bibliographically approved