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A complex gene locus enables xyloglucan utilization in the model saprophyte Cellvibrio japonicus
KTH, School of Biotechnology (BIO), Glycoscience.
KTH, School of Biotechnology (BIO), Glycoscience.ORCID iD: 0000-0002-7875-2822
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2014 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 94, no 2, 418-433 p.Article in journal (Refereed) Published
Abstract [en]

The degradation of plant biomass by saprophytes is an ecologically important part of the global carbon cycle, which has also inspired a vast diversity of industrial enzyme applications. The xyloglucans (XyGs) constitute a family of ubiquitous and abundant plant cell wall polysaccharides, yet the enzymology of XyG saccharification is poorly studied. Here, we present the identification and molecular characterization of a complex genetic locus that is required for xyloglucan utilization by the model saprophyte Cellvibrio japonicus. In harness, transcriptomics, reverse genetics, enzyme kinetics, and structural biology indicate that the encoded cohort of an -xylosidase, a -galactosidase, and an -l-fucosidase is specifically adapted for efficient, concerted saccharification of dicot (fucogalacto)xyloglucan oligosaccharides following import into the periplasm via an associated TonB-dependent receptor. The data support a biological model of xyloglucan degradation by C. japonicus with striking similarities - and notable differences - to the complex polysaccharide utilization loci of the Bacteroidetes.

Place, publisher, year, edition, pages
2014. Vol. 94, no 2, 418-433 p.
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:kth:diva-156455DOI: 10.1111/mmi.12776ISI: 000343755700013Scopus ID: 2-s2.0-84916926060OAI: oai:DiVA.org:kth-156455DiVA: diva2:767480
Note

QC 20141201

Available from: 2014-12-01 Created: 2014-11-28 Last updated: 2017-12-05Bibliographically approved

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Lundqvist, Magnus

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