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Detection of fluorescence spectra of individual bioaerosol particles
KTH, School of Engineering Sciences (SCI), Physics.
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2005 (English)In: Proc SPIE Int Soc Opt Eng, 2005Conference paper (Refereed)
Abstract [en]

We present initial results of a measurement system designed for detecting the fluorescence spectrum of individual particles of biological warfare agent (BWA). A compact optical parametric oscillator with intracavity sum-frequency mixing and a commercial Nitrogen gas laser was used as excitation sources to generate 293 nm or 337 nm UV laser irradiation. The pulsed lasers and a photomultiplier tube (PMT) array based spectrometer were triggered by a red laser-diode and a PMT detector that sensed the presence of a particle typical of size 5-20 μm in diameter. The spectral detection part of the system consisted of a grating and a PMT array with 32 channels, which measured fluorescence in the wavelength from 280 nm to 800 nm. The detector system was used to demonstrate the measurement of laser induced fluorescence spectra of individual BWA simulant particles by excitation of single UV laser pulses. The spectrum obtained by averaging spectra from several BWA aerosol simulant particles were found generally similar, but not identical, to the fluorescence spectrum obtained from water solutions containing the same particles dissolved.

Place, publisher, year, edition, pages
, Proceedings of SPIE - The International Society for Optical Engineering, ISSN 0277-786X ; 5990
Keyword [en]
Bioaerosol detection, Biosensing, Fluorescense spectroscopy, Point-detection, Ultraviolet laser excitation, Diffraction gratings, Fluorescence, Laser pulses, Optical parametric oscillators, Photomultipliers, Ultraviolet radiation, Biological warfare agent (BWA), Aerosols
National Category
Physical Sciences
URN: urn:nbn:se:kth:diva-156487DOI: 10.1117/12.630141ScopusID: 2-s2.0-33644509509OAI: diva2:768216
Optically Based Biological and Chemical Sensing, and Optically Based Materials for Defence, 28 September 2005, Bruges, Belgium

QC 20141203

Available from: 2014-12-03 Created: 2014-11-28 Last updated: 2014-12-03Bibliographically approved

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Tiihonen, Mikael
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