Change search
ReferencesLink to record
Permanent link

Direct link
Analysis of Autoantibody Profiles in Osteoarthritis Using Comprehensive Protein Array Concepts
KTH, Centres, Science for Life Laboratory, SciLifeLab.
Show others and affiliations
2014 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 13, no 11, 5218-5229 p.Article in journal (Refereed) Published
Abstract [en]

Osteoarthritis (OA) is the most common rheumatic disease and one of the most disabling pathologies worldwide. To date, the diagnostic methods of OA are very limited, and there are no available medications capable of halting its characteristic cartilage degeneration. Therefore, there is a significant interest in new biomarkers useful for the early diagnosis, prognosis, and therapeutic monitoring. In the recent years, protein microarrays have emerged as a powerful proteomic tool to search for new biomarkers. In this study, we have used two concepts for generating protein arrays, antigen microarrays, and NAPPA (nucleic acid programmable protein arrays), to characterize differential autoantibody profiles in a set of 62 samples from OA, rheumatoid arthritis (RA), and healthy controls. An untargeted screen was performed on 3840 protein fragments spotted on planar antigen arrays, and 373 antigens were selected for validation on bead-based arrays. In the NAPPA approach, a targeted screening was performed on 80 preselected proteins. The autoantibody targeting CHST14 was validated by ELISA in the same set of patients. Altogether, nine and seven disease related autoantibody target candidates were identified, and this work demonstrates a combination of these two array concepts for biomarker discovery and their usefulness for characterizing disease-specific autoantibody profiles.

Place, publisher, year, edition, pages
2014. Vol. 13, no 11, 5218-5229 p.
Keyword [en]
Protein microarrays, autoantibody profiles, osteoarthritis, NAPPA
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-157610DOI: 10.1021/pr500775aISI: 000344636500063ScopusID: 2-s2.0-84908888878OAI: diva2:771153
EU, FP7, Seventh Framework ProgrammeKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience

QC 20141212

Available from: 2014-12-12 Created: 2014-12-11 Last updated: 2014-12-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Henjes, FraukeSchwenk, Jochen M.Nilsson, Peter
By organisation
Science for Life Laboratory, SciLifeLabProteomics and Nanobiotechnology
In the same journal
Journal of Proteome Research
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 34 hits
ReferencesLink to record
Permanent link

Direct link