Microfluidic-based isolation of bacteria from whole blood for sepsis diagnostics
2015 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 37, no 4, 825-830 p.Article in journal (Refereed) Published
Blood-stream infections (BSI) remain a major health challenge, with an increasing incidence worldwide and a high mortality rate. Early treatment with appropriate antibiotics can reduce BSI-related morbidity and mortality, but success requires rapid identification of the infecting organisms. The rapid, culture-independent diagnosis of BSI could be significantly facilitated by straightforward isolation of highly purified bacteria from whole blood. We present a microfluidic-based, sample-preparation system that rapidly and selectively lyses all blood cells while it extracts intact bacteria for downstream analysis. Whole blood is exposed to a mild detergent, which lyses most blood cells, and then to osmotic shock using deionized water, which eliminates the remaining white blood cells. The recovered bacteria are 100 % viable, which opens up possibilities for performing drug susceptibility tests and for nucleic-acid-based molecular identification.
Place, publisher, year, edition, pages
2015. Vol. 37, no 4, 825-830 p.
Bacteria; Cells; Cytology; Deionized water; Microfluidics, Bacteria isolation; Blood streams; Cell lysis; Drug susceptibility; Isolation of bacteria; Microfluidic-based; Molecular identification; Rapid identification, Blood
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:kth:diva-157710DOI: 10.1007/s10529-014-1734-8ISI: 000351535300010ScopusID: 2-s2.0-84925535660OAI: oai:DiVA.org:kth-157710DiVA: diva2:771227
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201412122014-12-122014-12-122016-01-22Bibliographically approved