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Analysis and control of proteolysis of recombinant proteins in Escherichia coli.
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.
2004 (English)In: Advances in biochemical engineering/biotechnology, ISSN 0724-6145, Vol. 89, p. 163-195Article in journal (Refereed) Published
Abstract [en]

Proteolysis is one of the reasons for poor production of recombinant proteins in Escherichia coli. Important properties of E. coli proteases, which are relevant for the production of recombinant proteins, are reviewed. Furthermore, various strategies to control the proteolysis of the recombinant proteins are presented. These strategies for control of proteolysis can be applied on various stages of the process: design of more stable protein, a modification of the host cell in respect to proteolytic activity, optimisation of cultivation and downstream processing. However, before implementing these measures the proteolysis rate should be measured in order to calculate a potential benefit of reduced proteolysis rate.

Place, publisher, year, edition, pages
2004. Vol. 89, p. 163-195
Keywords [en]
bacterial protein, proteinase, recombinant protein, biosynthesis, culture technique, Escherichia coli, genetic enhancement, genetics, homeostasis, hydrolysis, metabolism, methodology, oxidative stress, physiology, protein engineering, review, Bacterial Proteins, Cell Culture Techniques, Endopeptidases, Recombinant Proteins
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:kth:diva-157426PubMedID: 15217159Scopus ID: 2-s2.0-3142689876OAI: oai:DiVA.org:kth-157426DiVA, id: diva2:771585
Note

QC 20141215

Available from: 2014-12-15 Created: 2014-12-09 Last updated: 2014-12-15Bibliographically approved

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Rozkov, Aleksei D.Enfors, Sven-Olof
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