Analysis and control of proteolysis of recombinant proteins in Escherichia coli.
2004 (English)In: Advances in biochemical engineering/biotechnology, ISSN 0724-6145, Vol. 89, 163-195 p.Article in journal (Refereed) Published
Proteolysis is one of the reasons for poor production of recombinant proteins in Escherichia coli. Important properties of E. coli proteases, which are relevant for the production of recombinant proteins, are reviewed. Furthermore, various strategies to control the proteolysis of the recombinant proteins are presented. These strategies for control of proteolysis can be applied on various stages of the process: design of more stable protein, a modification of the host cell in respect to proteolytic activity, optimisation of cultivation and downstream processing. However, before implementing these measures the proteolysis rate should be measured in order to calculate a potential benefit of reduced proteolysis rate.
Place, publisher, year, edition, pages
2004. Vol. 89, 163-195 p.
bacterial protein, proteinase, recombinant protein, biosynthesis, culture technique, Escherichia coli, genetic enhancement, genetics, homeostasis, hydrolysis, metabolism, methodology, oxidative stress, physiology, protein engineering, review, Bacterial Proteins, Cell Culture Techniques, Endopeptidases, Recombinant Proteins
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:kth:diva-157426PubMedID: 15217159ScopusID: 2-s2.0-3142689876OAI: oai:DiVA.org:kth-157426DiVA: diva2:771585
QC 201412152014-12-152014-12-092014-12-15Bibliographically approved