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Sensitive detection of C. parvum using near infrared emitting Ag2S@silica core-shell nanospheres
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
2014 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 4, no 107, 62399-62403 p.Article in journal (Refereed) Published
Abstract [en]

An optical immunosensor was developed using anti-oocysts McAb immobilized near-infrared (NIR) emitting Ag2S@silica core-shell nanospheres for the detection of C. parvum in water. The formation of the core Ag2S and the SiO2 shell over the core was confirmed by X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). The optical properties of the nanostructures were determined by UV-Vis spectroscopy and photoluminescence (PL) studies. The core-shell Ag2S@silica nanospheres exhibited an intense emission peak at 896 nm which falls in the biological window. TEM images confirm the presence of a uniform core-shell structure, with a Ag2S core with an average size of 80 nm, and a silica shell with a thickness of 40-50 nm. The C. parvum antibody anti-oocysts McAb immobilized Ag2S@silica nanoparticles were used as detector probes and these biosensors exhibited excellent analytical performance toward the detection of C. parvum, with detection limits of 10 oocysts per mL with a minimal assay period.

Place, publisher, year, edition, pages
2014. Vol. 4, no 107, 62399-62403 p.
Keyword [en]
Doped Silica Nanoparticles, Quantum Dots, Cryptosporidium-Parvum, Giardia, Water
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-158314DOI: 10.1039/c4ra10833gISI: 000345660300049Scopus ID: 2-s2.0-84912017627OAI: oai:DiVA.org:kth-158314DiVA: diva2:776138
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20150107

Available from: 2015-01-07 Created: 2015-01-07 Last updated: 2017-12-05Bibliographically approved

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