Sensitive detection of C. parvum using near infrared emitting Ag2S@silica core-shell nanospheres
2014 (English)In: RSC Advances, ISSN 2046-2069, Vol. 4, no 107, 62399-62403 p.Article in journal (Refereed) Published
An optical immunosensor was developed using anti-oocysts McAb immobilized near-infrared (NIR) emitting Ag2S@silica core-shell nanospheres for the detection of C. parvum in water. The formation of the core Ag2S and the SiO2 shell over the core was confirmed by X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). The optical properties of the nanostructures were determined by UV-Vis spectroscopy and photoluminescence (PL) studies. The core-shell Ag2S@silica nanospheres exhibited an intense emission peak at 896 nm which falls in the biological window. TEM images confirm the presence of a uniform core-shell structure, with a Ag2S core with an average size of 80 nm, and a silica shell with a thickness of 40-50 nm. The C. parvum antibody anti-oocysts McAb immobilized Ag2S@silica nanoparticles were used as detector probes and these biosensors exhibited excellent analytical performance toward the detection of C. parvum, with detection limits of 10 oocysts per mL with a minimal assay period.
Place, publisher, year, edition, pages
2014. Vol. 4, no 107, 62399-62403 p.
Doped Silica Nanoparticles, Quantum Dots, Cryptosporidium-Parvum, Giardia, Water
IdentifiersURN: urn:nbn:se:kth:diva-158314DOI: 10.1039/c4ra10833gISI: 000345660300049ScopusID: 2-s2.0-84912017627OAI: oai:DiVA.org:kth-158314DiVA: diva2:776138
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201501072015-01-072015-01-072015-01-07Bibliographically approved