Change search
ReferencesLink to record
Permanent link

Direct link
Design and synthesis of xyloglucan oligosaccharides: structure-function studies and application of xyloglucan endotransglycosylase PttXET16A
KTH, Superseded Departments, Biotechnology.
2004 (English)Licentiate thesis, comprehensive summary (Other scientific)
Abstract [en]

Primary cell walls are a composite of cellulose microfibrilsand hemicelluloses. Xyloglucan is the principal hemicelluloseof primary cell walls of dicotyledons. Xyloglucanendotransglycosylases (XETs) cleave and religate xyloglucanpolymers in plant cell walls. A XET (PttXET16A) from hybridaspen has been heterologously expressed and characterized inour lab.

To study XETs enzymology on a molecular level a series ofnovel xyloglucan oligosaccharides (XGOs) have been synthesized.The chromogenic 2-nitrophenol XGO and fluorogenic XGOs havebeen used as kinetic probes for PttXET16A. The first 3-Dstructure of the XET and of the enzyme-substrate complexrevealed new insights into the requirements fortransglycosylation.

Cellulose fibers are an important raw material for manyindustries. In a novel chemo-enzymatic approach, thetransglycosylating activity of XET was used for biomimeticfiber surface modification. The aminoalditol XGO derivate wasused as key intermediate to incorporate novel chemicalfunctionality into xyloglucan. TheXGO derivatives wereintegrated into xyloglucan with PttXET16A. The resultingmodified xyloglucan was used as a versatile tool fiber surfacemodification.

Place, publisher, year, edition, pages
Stockholm: Bioteknologi , 2004. , 53 p.
Keyword [en]
xyloglucan oligosaccharides, xyloglucan endotransglycosylase, XET, crystal structure, fiber surface modification, Populus tremula x Populus tremuloides, Hybrid aspen
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-1777ISBN: 91-7283-768-3OAI: diva2:7769
Available from: 2004-07-06 Created: 2004-07-06 Last updated: 2012-03-19Bibliographically approved

Open Access in DiVA

fulltext(894 kB)1309 downloads
File information
File name FULLTEXT01.pdfFile size 894 kBChecksum SHA-1
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Baumann, Martin J.
By organisation
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 1309 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 2209 hits
ReferencesLink to record
Permanent link

Direct link