Design and synthesis of xyloglucan oligosaccharides: structure-function studies and application of xyloglucan endotransglycosylase PttXET16A
2004 (English)Licentiate thesis, comprehensive summary (Other scientific)
Primary cell walls are a composite of cellulose microfibrilsand hemicelluloses. Xyloglucan is the principal hemicelluloseof primary cell walls of dicotyledons. Xyloglucanendotransglycosylases (XETs) cleave and religate xyloglucanpolymers in plant cell walls. A XET (PttXET16A) from hybridaspen has been heterologously expressed and characterized inour lab.
To study XETs enzymology on a molecular level a series ofnovel xyloglucan oligosaccharides (XGOs) have been synthesized.The chromogenic 2-nitrophenol XGO and fluorogenic XGOs havebeen used as kinetic probes for PttXET16A. The first 3-Dstructure of the XET and of the enzyme-substrate complexrevealed new insights into the requirements fortransglycosylation.
Cellulose fibers are an important raw material for manyindustries. In a novel chemo-enzymatic approach, thetransglycosylating activity of XET was used for biomimeticfiber surface modification. The aminoalditol XGO derivate wasused as key intermediate to incorporate novel chemicalfunctionality into xyloglucan. TheXGO derivatives wereintegrated into xyloglucan with PttXET16A. The resultingmodified xyloglucan was used as a versatile tool fiber surfacemodification.
Place, publisher, year, edition, pages
Stockholm: Bioteknologi , 2004. , 53 p.
xyloglucan oligosaccharides, xyloglucan endotransglycosylase, XET, crystal structure, fiber surface modification, Populus tremula x Populus tremuloides, Hybrid aspen
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-1777ISBN: 91-7283-768-3OAI: oai:DiVA.org:kth-1777DiVA: diva2:7769