Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Adsorption of Lysozyme on Silver and Its Influence on Silver Release
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0002-2123-2201
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2206-0082
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science. SP Technical Research Institute of Swede.ORCID iD: 0000-0001-7496-1101
2014 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 30, no 46, 13877-13889 p.Article in journal (Refereed) Published
Abstract [en]

Silver is increasingly used in antimicrobial coatings of biomedical devices and implants to hinder infections. As proteins have been shown to largely influence the extent of released metals from various metal surfaces at biological conditions, silver may also be influenced in the same way. The aim of this study is to relate the structure of adsorbed lysozyme (LSZ) to the release of silver from metallic silver surfaces. Simultaneous adsorption measurements were performed in real time on the same surface using combined ellipsometry and quartz crystal microbalance with dissipation monitoring measurements to provide a more comprehensive understanding on the adsorption kinetics and the layer structures. The concentration of LSZ in 0.15 M NaNO3 solution (pH 7, 25 degrees C) influences the structure of the adsorbed layer. Monolayer coverage is obtained at concentrations =0.1 g/L, while a bilayer structure with a rigid inner layer and a relatively loosely adsorbed outer layer is formed at 1 g/L. The inner layer of LSZ is assumed to bind firmly to silver via disulfide bridges, which makes it irreversibly adsorbed with respect to dilution. The amount of released silver is further influenced by the structure of the LSZ layer. At low LSZ concentrations (=0.1 g/L) the amount of released silver is not significantly different compared with non-protein-containing NaNO3 solutions; however, noticeable reduction was observed at higher concentrations (1 g/L). This reduction in silver release has several possible explanations, including (i) surface complexation between LSZ and silver ions that may result in the incorporation of silver in the irreversible adsorbed layer and, hence, reduce the amount of released silver into solution, and (ii) net charge reversal at the protein/solution interface to slightly positive surface potentials. Any release of silver will therefore exhibit an electrostatic repulsion during transportation through the protein layer results in a reduced amount of silver in solution.

Place, publisher, year, edition, pages
2014. Vol. 30, no 46, 13877-13889 p.
Keyword [en]
Adsorption, Covalent bonds, Crystallography, Enzymes, Metal ions, Quartz, Adsorption kinetics, Adsorption measurement, Antimicrobial coatings, Biological conditions, Biomedical devices, Electrostatic repulsion, Quartz crystal microbalance with dissipation monitoring, Surface complexation
National Category
Chemical Sciences Materials Engineering
Identifiers
URN: urn:nbn:se:kth:diva-158285DOI: 10.1021/la503170xISI: 000345552000020PubMedID: 25363360Scopus ID: 2-s2.0-84912573332OAI: oai:DiVA.org:kth-158285DiVA: diva2:777873
Funder
Swedish Research Council
Note

QC 20150109

Available from: 2015-01-09 Created: 2015-01-07 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Authority records BETA

Herting, GunillaOdnevall Wallinder, IngerBlomberg, Eva

Search in DiVA

By author/editor
Wang, XinHerting, GunillaOdnevall Wallinder, IngerBlomberg, Eva
By organisation
Surface and Corrosion Science
In the same journal
Langmuir
Chemical SciencesMaterials Engineering

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 31 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf