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Two-dimensional macromolecular distributions reveal detailed architectural features in high-amylose starches
KTH, School of Biotechnology (BIO), Glycoscience. Huazhong University of Science and Technology, China.ORCID iD: 0000-0003-3572-7798
2014 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 113, 539-551 p.Article in journal (Refereed) Published
Abstract [en]

Two-dimensional (2D) structural distributions based on macromolecular size and branch chain-length are obtained for three maize starches with different amylose contents (one normal and two high-amylose varieties). Data were obtained using an analytical methodology combining chemical fractionation, enzymatic debranching, and offline 2D size-exclusion chromatography with multiple detection. The 2D distributions reveal novel features in the branching structure of high-amylose maize starches. Normal maize starch shows well-resolved structural topologies, corresponding to the amylopectin and amylose macromolecular populations. However, high-amylose maize starches exhibit very complex topologies with significant features between those of amylose and amylopectin, showing the presence of distinct intermediate components. These have the macromolecular size of amylose but similar branching structure to amylopectin, except for a higher proportion of longer branches. These structural features of the intermediate components can be related to a less tightly controlled biosynthesis of the branching structures in high-amylose maize starch mutants, which may prevent these molecules from maturing into full-size amylopectin. This altered macromolecular branched architecture of high-amylose starches probably contribute to their better nutritional properties.

Place, publisher, year, edition, pages
2014. Vol. 113, 539-551 p.
Keyword [en]
Branching, Size-exclusion chromatography, Starch, Amylose, Amylopectin, Intermediate components
National Category
Chemical Sciences
URN: urn:nbn:se:kth:diva-156102DOI: 10.1016/j.carbpol.2014.07.050ISI: 000343613000067ScopusID: 2-s2.0-84907303916OAI: diva2:778152
Knut and Alice Wallenberg Foundation

QC 20150109

Available from: 2015-01-09 Created: 2014-11-21 Last updated: 2015-01-09Bibliographically approved

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