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Superhydrophobic MALDI Concentration Plates for Improved Analysis of Butterfly Reproduction Proteins Using Capillary Electrophoresis and MALDI-TOF/TOF
KTH, School of Chemical Science and Engineering (CHE).
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Superhydrofoba MALDI-koncentreringsplattor för förbättrad analys av fjärilsreproduktionsproteiner med kapillärelektrofores och MALDI-TOF/TOF (Swedish)
Abstract [en]

To analyze reproduction proteins from the butterfly Pieris Napi (green-veined white butterfly) a method has been developed with capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in earlier works. Since the sample volumes are small, as often with biological samples, there is a necessity to use MALDI concentration plates. When coupling MALDI with CE it has been shown that the surface of the MALDI concentration plate gets destroyed during the process. To reduce costs, previous works have been using silicone coated MALDI concentration plates, which needs a cleaning process involving the harmful solvent hexane.  To  reduce the total impact on the environment and health, a new MALDI concentration plate was developed. There was also a requirement for the MALDI concentration plate to be cheap and easy to produce. Alkyl ketene dimer (AKD) was used to produce superhydrophobic MALDI concentration plates in the present work. Various techniques were tested to make the AKD surface, and two of the techniques were successful, air brushing and rapid expansion of supercritical solvent (RESS), with RESS as the strongest candidate. When manually applying samples on the AKD MALDI concentration plate there was no observed contamination of the sample, and there were more “sweet spots” than when using a regular MALDI plate. There were extra peaks, caused by the CE-fractioning, observed when analyzing the fractions from the CE, which correlates to previous works. However, it was possible to study fractions from aqueous extracts from P.Napi and fragment some interesting peaks by using MALDI-TOF/TOF. Unfortunately the peptide database was not accessible at the time being and proteins and peptides could not be identified.

Place, publisher, year, edition, pages
2014.
Keyword [en]
Superhydrophobicity, Alkyl ketene dimer, Pieris Napi, Capillary electrophoresis, , MALDI
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-159170OAI: oai:DiVA.org:kth-159170DiVA: diva2:782777
Available from: 2015-01-22 Created: 2015-01-22 Last updated: 2017-11-27Bibliographically approved

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