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The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling
KTH, Centres, Science for Life Laboratory, SciLifeLab. Karolinska University Hospital, Sweden .
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-0198-7137
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 12, e116125- p.Article in journal (Refereed) Published
Abstract [en]

To understand renal functions and disease, it is important to define the molecular constituents of the various compartments of the kidney. Here, we used comparative transcriptomic analysis of all major organs and tissues in the human body, in combination with kidney tissue micro array based immunohistochemistry, to generate a comprehensive description of the kidney-specific transcriptome and proteome. A special emphasis was placed on the identification of genes and proteins that were elevated in specific kidney subcompartments. Our analysis identified close to 400 genes that had elevated expression in the kidney, as compared to the other analysed tissues, and these were further subdivided, depending on expression levels, into tissue enriched, group enriched or tissue enhanced. Immunohistochemistry allowed us to identify proteins with distinct localisation to the glomeruli (n=11), proximal tubules (n=120), distal tubules (n=9) or collecting ducts (n=8). Among the identified kidney elevated transcripts, we found several proteins not previously characterised or identified as elevated in kidney. This description of the kidney specific transcriptome and proteome provides a resource for basic and clinical research to facilitate studies to understand kidney biology and disease.

Place, publisher, year, edition, pages
2014. Vol. 9, no 12, e116125- p.
Keyword [en]
Alpha-Crystallin, Slit Diaphragm, Rna-Seq, Gene, Nephrin, Expression, Disease, Biology, Tool, Uromodulin
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URN: urn:nbn:se:kth:diva-159369DOI: 10.1371/journal.pone.0116125ISI: 000347119100096Scopus ID: 2-s2.0-84920434621OAI: oai:DiVA.org:kth-159369DiVA: diva2:784708
Funder
Knut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20150130

Available from: 2015-01-30 Created: 2015-01-29 Last updated: 2017-12-05Bibliographically approved

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Fagerberg, LinnUhlén, Mathias

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