Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).ORCID iD: 0000-0002-9327-2544
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).
Show others and affiliations
2005 (English)In: Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Transducers Research Foundations, 2005, 1006-1008 p.Conference paper, Published paper (Refereed)
Abstract [en]

This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis.

Place, publisher, year, edition, pages
Transducers Research Foundations, 2005. 1006-1008 p.
Keyword [en]
Beads, DASH, Microheater, Single nucleotide polymorphism
National Category
Other Engineering and Technologies
Identifiers
URN: urn:nbn:se:kth:diva-91800Scopus ID: 2-s2.0-84902494047ISBN: 978-097436111-6 (print)OAI: oai:DiVA.org:kth-91800DiVA: diva2:784943
Conference
9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 9 October 2005 through 13 October 2005
Note

QC 20150206

Available from: 2015-01-31 Created: 2012-03-20 Last updated: 2015-02-06Bibliographically approved

Open Access in DiVA

No full text

Scopus

Authority records BETA

Haasl, SjoerdStemme, Göran

Search in DiVA

By author/editor
Russom, AmanHaasl, SjoerdAndersson, HeleneStemme, Göran
By organisation
Microsystem Technology (Changed name 20121201)
Other Engineering and Technologies

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 9 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf