Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism
2005 (English)In: Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Transducers Research Foundations, 2005, 1006-1008 p.Conference paper (Refereed)
This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis.
Place, publisher, year, edition, pages
Transducers Research Foundations, 2005. 1006-1008 p.
Beads, DASH, Microheater, Single nucleotide polymorphism
Other Engineering and Technologies
IdentifiersURN: urn:nbn:se:kth:diva-91800ScopusID: 2-s2.0-84902494047ISBN: 978-097436111-6OAI: oai:DiVA.org:kth-91800DiVA: diva2:784943
9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 9 October 2005 through 13 October 2005
QC 201502062015-01-312012-03-202015-02-06Bibliographically approved