Yeast extract from express five serum-free medium contains factors at about 35 kDa, essential for growth of Trichoplusia ni insect cells
2005 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 27, no 20, 1623-1627 p.Article in journal (Refereed) Published
The yeast extract (of unknown origin) present in the commercially available serum-free medium 'Express Five' contains factors ('yeast extract factors') up to 35 kDa which are essential for growth of Trichoplusia ni insect cells. A yeast extract brand lacking these components could not support growth of T. ni cells. However, cell proliferation was restored by adding chromatographic fractions containing the yeast extract factors. The yeast extract factors were not solely responsible for the growth enhancing effect of yeast extract but some other components, which seem to be generally present in yeast extracts, are also required for T. ni proliferation.
Place, publisher, year, edition, pages
2005. Vol. 27, no 20, 1623-1627 p.
Essential nutrients, Express Five, Insect cells, Serum-free medium, Trichoplusia ni, Yeast extract
IdentifiersURN: urn:nbn:se:kth:diva-5095DOI: 10.1007/s10529-005-2603-2ISI: 000232799300014OAI: oai:DiVA.org:kth-5095DiVA: diva2:7855
QC 20101005. Uppdaterad från Manuskript till Artikel (20101005). Tidigare Titel:"Yeast extract from Express Five SFM contains essential factors for growth of Trichoplusia ni insect cells".