Single-step manufacturing of femtoliter microwell arrays in a novel surface energy mimicking polymer
2015 (English)In: 18th International Conference on Solid-State Sensors, Actuators and Microsystems (IEEE TRANSDUCER 2015), IEEE , 2015Conference paper, Abstract (Refereed)
We report a novel polymer material formulation and stamp-molding technique that enable rapid single-step manufacturing of hydrophilic-in-hydrophobic microwell arrays. We developed a modified thiol-ene-epoxy polymer (mOSTE+) formulation that mimics the surface energy of its mold during polymerization. The polymer inherits the surface energy from the mold through molecular self-assembly, in which functional monomers self-assemble at the interface between the liquid prepolymer and the mold surface. Combining this novel mOSTE+ material with a stamp-molding process leads to simultaneous surface energy mimicking and micro-structuring. This method was used to manufacture microwells with hydrophilic bottom and hydrophobic sidewall, depressed in a surrounding hydrophobic surface. The microwell arrays were successfully tested for the self-assembly of 62’000 femtoliter-droplets. Such femtoliter droplet arrays are useful for, e.g., digital ELISA and single cell/molecule analysis applications.
Place, publisher, year, edition, pages
IEEE , 2015.
OSTE, microarray, bioassay, biosensor, diagnostics, lab-on-chip, loc, microfluidics, OSTEmer, surface modification, microwell, microwell array
Nano Technology Other Electrical Engineering, Electronic Engineering, Information Engineering Textile, Rubber and Polymeric Materials Medical Laboratory and Measurements Technologies Polymer Technologies
Research subject Materials Science and Engineering; Electrical Engineering; Biotechnology; Fibre and Polymer Science; Medical Technology
IdentifiersURN: urn:nbn:se:kth:diva-159580ISI: 000380461400128ScopusID: 2-s2.0-84955439055OAI: oai:DiVA.org:kth-159580DiVA: diva2:785864
18th International Conference on Solid-State Sensors, Actuators and Microsystems (IEEE TRANSDUCER 2015), Alaska, USA, 21-25 Jun, 2015
QC 201502102015-02-042015-02-042016-09-02Bibliographically approved