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Microfluidic force spectroscopy for characterization of biomolecular interactions with piconewton resolution
Stanford Genome Technology Center, Stanford University, United States.
2010 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 97, no 17, 173704- p.Article in journal (Refereed) Published
Abstract [en]

In this paper we present a scalable method based on the use of microfluidics and shear force spectroscopy which can be used for determining the affinity between molecules. Our method involves the use of functionalization of the surface of microfluidic channels with ligand molecules, and the surface of microspheres with receptor molecules. Bound beads are detached from the surface of the microchannels using pressure driven flow. The drag force required to detach the beads is used to determine the affinity of the bond holding the two molecules together. The minimum force we are able to detect is 5 pN. We have used this method to determine the binding force between protein-protein interactions and DNA base-pair interactions. We also have shown the ability of this technique to distinguish between strong and weak protein-protein interactions. Using this approach, it may be possible to multiplex an array of these functionalized channels onto a chip and probe the interactions between large varieties of biomolecules.

Place, publisher, year, edition, pages
2010. Vol. 97, no 17, 173704- p.
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-159923DOI: 10.1063/1.3491547ISI: 000284233600072Scopus ID: 2-s2.0-78149431420OAI: oai:DiVA.org:kth-159923DiVA: diva2:787877
Note

QC 20150212

Available from: 2015-02-12 Created: 2015-02-12 Last updated: 2017-12-04Bibliographically approved

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