An orthogonal fusion tag for efficient protein purification
2014 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1129, 205-210 p.Article in journal (Refereed) Published
Protein fusion tags are important tools in research when robust methods for protein purification and detection are required. In this chapter we present an efficient method for stringent protein purification. A small domain, denoted ABDz1, with affinity for both human serum albumin and Protein A has been developed. The purification tag is based on an albumin-binding domain from Streptococcal Protein G that was engineered to bind Protein A. The ABDz1-tag can be fused to any protein of choice and the purification can be performed using standard laboratory equipment. In this chapter a method for purification of ABDz1-tagged proteins using two successive affinity purification steps is described.
Place, publisher, year, edition, pages
2014. Vol. 1129, 205-210 p.
affinity chromatography, article, isolation and purification, methodology, protein binding
IdentifiersURN: urn:nbn:se:kth:diva-161684DOI: 10.1007/978-1-62703-977-2_18PubMedID: 24648079ScopusID: 2-s2.0-84909987778OAI: oai:DiVA.org:kth-161684DiVA: diva2:795450
QC 201503162015-03-162015-03-132015-03-16Bibliographically approved