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An orthogonal fusion tag for efficient protein purification
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0002-6104-6446
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0003-4008-5275
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0003-0605-8417
2014 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1129, 205-210 p.Article in journal (Refereed) Published
Abstract [en]

Protein fusion tags are important tools in research when robust methods for protein purification and detection are required. In this chapter we present an efficient method for stringent protein purification. A small domain, denoted ABDz1, with affinity for both human serum albumin and Protein A has been developed. The purification tag is based on an albumin-binding domain from Streptococcal Protein G that was engineered to bind Protein A. The ABDz1-tag can be fused to any protein of choice and the purification can be performed using standard laboratory equipment. In this chapter a method for purification of ABDz1-tagged proteins using two successive affinity purification steps is described.

Place, publisher, year, edition, pages
2014. Vol. 1129, 205-210 p.
Keyword [en]
affinity chromatography, article, isolation and purification, methodology, protein binding
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-161684DOI: 10.1007/978-1-62703-977-2_18PubMedID: 24648079Scopus ID: 2-s2.0-84909987778OAI: oai:DiVA.org:kth-161684DiVA: diva2:795450
Note

QC 20150316

Available from: 2015-03-16 Created: 2015-03-13 Last updated: 2017-12-04Bibliographically approved

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Nilvebrant, JohanÅstrand, MikaelHober, Sophia

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