Removal of viruses, parasitic protozoa microbial indicators and correlation with process indicators in conventional and membrane processes in a wastewater pilot plant
2006 (English)In: Water Research, ISSN 0043-1354, E-ISSN 1879-2448, Vol. 40, no 7, 1449-1457 p.Article in journal (Refereed) Published
The aim of this study was to investigate variations in the occurrence and removal of enterovirus and norovirus genomes, Giardia cysts, Cryptosporidium oocysts and the most commonly used faecal indicators in a Swedish wastewater pilot plant. Paired samples were taken from the inlet and outlet of each treatment line: tertiary filtration, membrane bioreactor (MBR) and upflow anaerobic sludge blankets (UASB). (Oo)cysts and indicators were enumerated using standard methods and viruses using RT-PCR. Giardia cysts and enteroviruses were constantly detected, mean numbers 10(3.11) cysts and 10(4.0) PCR units L-1, respectively. Oocysts were found in 5/19 samples, mean number 5 L-1. Noroviruses were found in 6/7 influent samples, with an average titre of 10(3 28) L-1, during winter, but only in 2/15 in the rest of the year (mean 200 L-1). MBR treatment removed indicators more efficiently than did the other two lines, with 5 log removal of E. coli. Human virus genome removal did not differ between the MBR and tertiary treatment line. Microorganism removal in UASB was significantly lower for all the organisms studied. E. coli, enterococci and Cl. perfringens removal was correlated (p < 0.05) with enterovirus genome removal, with R-values around 0.4. However, values for removal of indicators were more strongly correlated to each other. Removal of viruses based on enumeration using RT-PCR probably underestimates infectious virion removal.
Place, publisher, year, edition, pages
2006. Vol. 40, no 7, 1449-1457 p.
wastewater treatment, MBR, UASB, enterovirus, norovirus, PCR, Giardia cysts, Cryptosporidium oocyst, faecal indicators, removal, phages
IdentifiersURN: urn:nbn:se:kth:diva-5182DOI: 10.1016/j.watres.2006.01.039ISI: 000236840800014ScopusID: 2-s2.0-33645112004OAI: oai:DiVA.org:kth-5182DiVA: diva2:7992
QC 20101013. Updated from manuscript to article in journal.2005-05-032005-05-032012-03-13Bibliographically approved