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Tillämpning av tre olika typer av perfusionsodlingssystem
KTH, School of Biotechnology (BIO).
2014 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Applications of three different systems for perfusion cultivation (English)
Abstract [en]

This work is focused in the study of three different ways of perfusion. The spin tube system was used in order to see metabolic differences between commercial cultivation media for CHO culture. A preliminary selection was carried out by vector analysis. This method was proposed in order to select the media with most singular features. 11 media were selected for a 12-day SpinTube experiment. Some correlations between matabolic rates were established. The media presenting the most unique characteristics were tested in STR experiment: acoustic settler and ATF. The first one required parameter adjustments to obtain high cell densities. Dut to time constrains, the filtration system was switxhed to ATF. Both systems were connected to the bioreactor with dip tube ended by a U-shaped profile, which jeopardized the operation due to mis-dimensioning. Two media could be analyzed and a correlation between STR and SpinTube sysstems was established. Finally, a small bioreactor prototype for stem cell culture was used. A useful system was designed and mounted for this prototype. Afterwards, a functional testing was carried out and some issues regarding temperature, pH, evaporation, and leakage were detected. Some solutions were proposed and put into practice, which contributed to the improvement and development of this promising prototype.

Abstract [sv]

Detta arbete fokuserade på tre alternativa säätt att genomföra perfusion. Systemet Spintube användes för att detektera metabola skillnader mellan kommersiella odlingsmedia för CHO celler. Ett preliminärt urval genomfördes med hjälp av vektoranalys, för att hitta de media med mest skilda egenskaper. 11 media valdes ut till ett 12-dagars SpinTube experiment. Några korrelationer mellan metabola hastigheter etablerades. De media som hade mest unika egenskaper valdes till ett STR perfusion experiment. Två filgrationssystem testades under experimentet: acoustic settler och ATF. I det först nämnda behövdes parameterjusteringar för att nå höga celldensiteter. På grund av tidsbrist, byttes filtrationssystem till ATF. Båda systemen kopplades till bioreaktrn med ett stigrör som avslutades med en U-form profil, vilket äventyrade oeprationen på grund av feldimensionering. Två media kunde analyseras och en korrelation mellan STR och SpinTubesystem etablerades. Slutligen användes en liten biorektorprototyp för stammcellsodling. Ett användbart system togs fram och byggdes upp flr denna prototyp. Efteråt utfördes ett funktionellt test där några problem identifierades: temperatur, pH, avdunstning och läckage. Några lösningar togs fram och testades vilket möjliggjorde en förbättring och utvecklande av en lovande prototyp.

Place, publisher, year, edition, pages
2014.
Keyword [en]
perfusion, stem cell culture, recombinant protein production, stirred tank reactor, spin tubes
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-163672OAI: oai:DiVA.org:kth-163672DiVA: diva2:801733
Supervisors
Examiners
Available from: 2015-04-14 Created: 2015-04-10 Last updated: 2015-09-15Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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  • vancouver
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  • de-DE
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  • en-US
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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