Projection - specific connectivity mapping of serotonergic neurons in the dorsal raphe
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Genetic engineering has ushered a new paradigm in neuroscience research. The possibilities that this confers to research methodologies is in large limited only by the supply of innovative ideas. The hope is that some of these new approaches will aid in the unfolding of the mechanistics behind brain function. The advantages include the possibility to utilize the difference in the genomic structure of specific cell-types to exclusively examine defined neuronal populations. This master's thesis implements a new approach to genetically based connectivity mapping that includes transgenic mice and viral vectors. The presented "projection specific" approach maps the origins of the monosynaptic inputs to the serotenergic neurons of the dorsal raphe (DR) mucleus that have axonal projections to either the prefrontal cortex (PFC) or the striatum (STR). The methodology is explained and expermental data ispresented. Conclusions include the validity of the methodology as a potential apprach to connectivity mapping that coould provide insights specific from other methods. However, variability in results and used reagents, as well as failed experimental trials, led to data of insufficient statistical power to confer conclusions regarding connectivity patterns. Bottlenecks and future improvements of the presented protocol are further discussed.
Place, publisher, year, edition, pages
Neuron, rabies virus, Adeno-associated virus, brain dorsal raphe, prefrontal cortex, striatum
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-163681OAI: oai:DiVA.org:kth-163681DiVA: diva2:801785