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Karakterisering av ett nytt metaboliskt enzym som är kritiskt för tillväxt av cancerceller
KTH, School of Biotechnology (BIO).
2014 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Characterization of a novel metabolic enzyme critical for cancer cell proliferation (English)
Abstract [en]

An altered cancer cell metabolis is today regarded as a hallmark of cancer and several metabolic pathways, including the one-carbon metabolism, are being investigated for their role in cancerl cell transformation. Recent findins have shown that one of the metabolic enzymes of the one-carbon metabolism is upregulated ina number of different cancer tumors, but is absen in most normal adult tissues. Furthermore, this metabolic enzyme has been demonstrated to have an importatnt role in suporting cancer cell proliferation and survival, but the mechanism behind this remains unclear. Here, we aim to characterize the role of this metabolic enzyme in cancer cell prolifertion and survival in order to gain a deeper understanding of the enzyme for its future use as a potential therapeutical terget. First, we show evidence that the enzyme localizes to the nucleus using confocal microscopy in tow cancer cell lines of different origin. Secondly, we were able to demonstrate an increased cellular proliferation when overexpressing the enzyme in cancer cell lines and by that validate its role in supporting cancer cell proliferation. Thirdly, we find that overexpression of the protein stimulates cancer cell proliferation independent of its enzymatoc activity. Taken togther, we here provid important data that will aid in future evaluation of the enzyme as a potential drug target in cancer treatment. 

Place, publisher, year, edition, pages
Keyword [en]
Cancer, Metabolic enzymes, tumor therapy, one-carbon metabolism, folate
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-163693OAI: diva2:801905
Available from: 2015-04-13 Created: 2015-04-10 Last updated: 2015-09-14Bibliographically approved

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