Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium
1995 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 369, no 2-3, 233-238 p.Article in journal (Refereed) Published
The cDNA of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium has been cloned and sequenced. The 5′ end was obtained by PCR amplification. The cDNA contains 2310 translated bases excluding the poly(A) tail. The deduced mature protein contains 770 amino acid residues and is preceded by a 18 residue long signal peptide. The regions of the amino acid sequence corresponding to the heme and FAD domains of CDH were identified as well as the nucleotide-binding motif, the disulfide pairing and a methionine residue chelating the heme iron. No homologous sequences were found for the heme domain, however, the FAD domain appears to be distantly related to the GMC oxidoreductase family.
Place, publisher, year, edition, pages
1995. Vol. 369, no 2-3, 233-238 p.
Cellobiose dehydrogenase, FAD, Heme, Nucleotide sequence, Nucleotide-binding motif, Wood degradation
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-164148DOI: 10.1016/0014-5793(95)00758-2ISI: A1995RP17600024PubMedID: 7649263ScopusID: 2-s2.0-0029085553OAI: oai:DiVA.org:kth-164148DiVA: diva2:803829
QC 201504142015-04-132015-04-132015-04-14Bibliographically approved