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Development of an on-site lateral flow immunoassay as a diagnostic tool for contagious bovinen pleuropneumonia
KTH, School of Biotechnology (BIO).
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Contagious bovine pleuropneumonia is a severe lung disease effecting cattle that is enzootic to Sub-Saharan Africa. No satisfactory vaccine or treatment is yet available for treatment of this infectious disease caused by the bacterial pathogen Mycoplasma mycoides subsp. mycoides (Mmm), which leads to its continuous spread over the continent with large socio-economic consequences as a result. By developing a diagnostic tool that is independent from laboratory equipment progress in eradicating this disease in the developing world can be made. In this investigation, construction of a lateral flow protein microarray with recombinant Mmm surface proteins was conducted and each step in the lateral flow assay was evaluated. A field trip to International Livestock Research Institue in Kenya was made in order to evaluate the prototype. The point-of-care prototype could discriminate between infected a non-infected cattle, with a sensitivity of 100% and specificity of 65%.

Place, publisher, year, edition, pages
2014.
Keyword [en]
CBPP, diagnostics, immunoassay, lateral flow, recombinant protein
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-164243OAI: oai:DiVA.org:kth-164243DiVA: diva2:804984
Supervisors
Examiners
Available from: 2015-04-15 Created: 2015-04-14 Last updated: 2015-09-17Bibliographically approved

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