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Method for labeling of compounds
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0003-0605-8417
KTH, School of Biotechnology (BIO), Protein Technology.ORCID iD: 0000-0002-0695-5188
2010 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The present invention relates to site-specific labeling of antibodies or fragments thereof with one or more reporter group(s) in a way that does not affect antigen binding. The method for labeling antibodies and/or fragments thereof, comprises the following steps a) providing an IgG binding protein, which comprises [alpha]-helix structures, with a photoactivatable group and at least one label; b) forming a mixture of said IgG binding protein and the antibodies and/or fragments to be labeled; and c) UV illuminating said mixture for site-specific labeling of said antibodies and/or fragments thereof. The IgG binding protein is preferably the Z domain of Protein A.

Place, publisher, year, edition, pages
2010.
National Category
Medical and Health Sciences
Research subject
Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-164247OAI: oai:DiVA.org:kth-164247DiVA: diva2:805011
Patent
US 8916689B2 (2014-12-23)
Note

QC 20150417

Available from: 2015-04-14 Created: 2015-04-14 Last updated: 2015-04-17Bibliographically approved

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Hober, SophiaEriksson Karlström, Amelie

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