PCR amplification and genetic analysis in a microwell cell cultivation chip
2008 (English)In: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, Chemical and Biological Microsystems Society , 2008, 576-578 p.Conference paper (Refereed)
We present a method for long-term single cell/clone cultivation followed by cell lysis, DNA amplification and detection of PCR product in a chip containing 672 individual microwells. By performing all steps on-chip in microwells, the proliferation and cell morphology of every single cell or clone can be linked to its genetic information. In this study two mammalian cell lines (mutated A431 vs. wild type U-2 OS) were used as a model system for mutation screening in the p53 gene. The presented method could improve the sensitivity in mutation frequency analysis of heterogeneous tumor samples.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2008. 576-578 p.
Clonal analysis, Mutation screening, PCR, Single cell
IdentifiersURN: urn:nbn:se:kth:diva-164359ScopusID: 2-s2.0-84902436871OAI: oai:DiVA.org:kth-164359DiVA: diva2:805780
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, 12 October 2008 through 16 October 2008, San Diego, CA, United States
QC 201504162015-04-162015-04-162015-04-16Bibliographically approved