Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
PCR amplification and genetic analysis in a microwell cell cultivation chip
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Gene Technology.
Show others and affiliations
2008 (English)In: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, Chemical and Biological Microsystems Society , 2008, 576-578 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present a method for long-term single cell/clone cultivation followed by cell lysis, DNA amplification and detection of PCR product in a chip containing 672 individual microwells. By performing all steps on-chip in microwells, the proliferation and cell morphology of every single cell or clone can be linked to its genetic information. In this study two mammalian cell lines (mutated A431 vs. wild type U-2 OS) were used as a model system for mutation screening in the p53 gene. The presented method could improve the sensitivity in mutation frequency analysis of heterogeneous tumor samples.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2008. 576-578 p.
Keyword [en]
Clonal analysis, Mutation screening, PCR, Single cell
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-164359Scopus ID: 2-s2.0-84902436871OAI: oai:DiVA.org:kth-164359DiVA: diva2:805780
Conference
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, 12 October 2008 through 16 October 2008, San Diego, CA, United States
Note

QC 20150416

Available from: 2015-04-16 Created: 2015-04-16 Last updated: 2015-04-16Bibliographically approved

Open Access in DiVA

No full text

Scopus

Search in DiVA

By author/editor
Lindström, SaraHammond, MariaGantelius, JesperAhmadian, AfshinAndersson-Svahn, Helene
By organisation
Nano Biotechnology (closed 20130101)Gene Technology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 14 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf