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Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).ORCID iD: 0000-0001-5232-0805
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2008 (English)In: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference, Chemical and Biological Microsystems Society , 2008, 1287-1289 p.Conference paper, Published paper (Refereed)
Abstract [en]

We have developed a novel microfluidic droplet based assay for analysis of low expressed cell surface proteins on individual cells at rates of hundreds of cells/s by antibody coupled enzymatic amplification in monodisperse droplets [1]. Here we expand the method to include concurrent analysis of multiple populations of single cells. We report the validation of the method by analyzing the human monocytic cell line U937 for two low expressed markers, CCR5 and CD19. Comparing our method to standard flow cytometry, we demonstrate increased peak separation, which should allow sorting by these low expressed biomarkers unavailable to flow cytometry.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2008. 1287-1289 p.
Keyword [en]
Antibodies, Cell surface biomarkers, Droplets, Enzymes
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-164351Scopus ID: 2-s2.0-84902507762OAI: oai:DiVA.org:kth-164351DiVA: diva2:805801
Conference
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, 12 October 2008 through 16 October 2008, San Diego, CA, United States
Note

QC 20150416

Available from: 2015-04-16 Created: 2015-04-16 Last updated: 2015-04-16Bibliographically approved

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Joensson, HaakanUhlén, Mathias

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Joensson, HaakanUhlén, MathiasAndersson Svahn, Helene
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