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Cross-link debonding in actin networks: influence on mechanical properties
KTH, School of Engineering Sciences (SCI), Solid Mechanics (Dept.), Solid Mechanics (Div.).ORCID iD: 0000-0002-6388-0995
KTH, School of Engineering Sciences (SCI), Solid Mechanics (Dept.), Solid Mechanics (Div.).ORCID iD: 0000-0003-3611-2250
KTH, School of Engineering Sciences (SCI), Solid Mechanics (Dept.), Solid Mechanics (Div.).
2015 (English)In: International Journal of Experimental and Computational Biomechanics, ISSN 1755-8743, Vol. 3, no 1, 16-26 p., b778558v5j17h4n8Article in journal (Refereed) Published
Abstract [en]

The actin cytoskeleton is essential for the continued function and survival of the cell. A peculiar mechanical characteristic of actin networks is their remodelling ability, providing them with a time-dependent response to mechanical forces. In cross-linked actin networks, this behaviour is typically tuned by the binding affinity of the cross-link. We propose that the debonding of a cross-link between filaments can be modelled using a stochastic approach, in which the activation energy for a bond is modified by a term to account for mechanical strain energy. By use of a finite element model, we perform numerical analyses in which we first compare the model behaviour to experimental results. The computed and experimental results are in good agreement for short time scales, but over longer time scales the stress is overestimated. However, it does provide a possible explanation for experimentally observed strain-rate dependence as well as strain-softening at longer time scales.

Place, publisher, year, edition, pages
2015. Vol. 3, no 1, 16-26 p., b778558v5j17h4n8
National Category
Applied Mechanics
Research subject
Solid Mechanics
Identifiers
URN: urn:nbn:se:kth:diva-164408DOI: 10.1504/IJECB.2015.067679OAI: oai:DiVA.org:kth-164408DiVA: diva2:805889
Note

QC 20150506. QC 20160211. QC 20160314

Available from: 2015-04-16 Created: 2015-04-16 Last updated: 2016-03-14Bibliographically approved
In thesis
1. On the mechanics of actin and intermediate filament networks and their contribution to cellular mechanics
Open this publication in new window or tab >>On the mechanics of actin and intermediate filament networks and their contribution to cellular mechanics
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The mechanical behaviour of cells is essential in ensuring continued physiological function, and deficiencies therein can result in a variety of diseases. Also, altered mechanical response of cells can in certain cases be an indicator of a diseased state, and even actively promoting progression of pathology. In this thesis, methods to model cell and cytoskeletal mechanics are developed and analysed.

In Paper A, a constitutive model for the response of transiently cross-linked actin networks is developed using a continuum framework. A strain energy function is proposed and modified in terms of chemically activated cross-links.

In Paper B, a finite element framework was used to assess the influence of numerous geometrical and material parameters on the response of cross-linked actin networks, quantifying the influence of microstructural properties and cross-link compliance. Also, a micromechanically motivated constitutive model for cross-linked networks in a continuum framework was proposed.

In Paper C, the discrete model is extended to include the stochastic nature of cross-links. The strain rate dependence observed in experiments is suggested to depend partly on this.

In Paper D, the continuum model for cross-linked networks is extended to encompass more composite networks. Favourable comparisons to experiments indicate the interplay between phenomenological evolution laws to predict effects in biopolymer networks.

In Paper E, experimental and computational techniques are used to assess influence of the actin cytoskeleton on the mechanical response of fibroblast cells. The influence of cell shape is assessed, and experimental and computational aspects of cell mechanics are discussed.

In Paper F, the filament-based cytoskeletal model is extended with an active response to predict active force generation.  Importantly, experimentally observed stiffening of cells with applied stress is predicted.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2015. 68 p.
Series
TRITA-HFL. Report / Royal Institute of Technology, Solid Mechanics, ISSN 1654-1472 ; 0583
Keyword
actin, cell, mechanical, constitutive, intermediate, continuum, constitutive
National Category
Applied Mechanics
Research subject
Solid Mechanics
Identifiers
urn:nbn:se:kth:diva-175748 (URN)978-91-7595-752-4 (ISBN)
Public defence
2016-01-29, Kollegiesalen, Brinellvägen 8, Stockholm, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council, A0437201
Note

QC 20151209

Available from: 2015-12-09 Created: 2015-10-20 Last updated: 2015-12-09Bibliographically approved

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Publisher's full texthttp://inderscience.metapress.com/content/b778558v5j17h4n8/

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Fallqvist, BjörnKulachenko, Artem

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