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Graft linker immobilization for spatial control of antibody immobilization inside fused microchips
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
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2009 (English)In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2009, 1566-1568 p.Conference paper, Published paper (Refereed)
Abstract [en]

A new method for antibody immobilization to the inside of fused silica microchips coated with a protein repelling polymer (2-methacryloyloxyethyl phosphorylcholine polymer, MPC polymer) is described. Antibody patterning is achieved by using a new photoreactive linker which is composed of three-parts: a carboxyl group (-COOH), a hydrophilic PEG spacer and a photoreactive benzophenone. The linker is grafted with UV which introduces free carboxyl groups to the microchannel surface. Antibodies are immobilized to these areas, by using amide bonding between protein -NH2's and linker's -COOH. Patterned anti-BSA antibodies retained functionality and were capable of antigen specific capture.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2009. 1566-1568 p.
Keyword [en]
Biosensors, Chemical bonds, Grafting (chemical), Microprocessor chips, Photoreactivity, Polymers, 2-Methacryloyloxyethyl phosphorylcholine polymers, Antibody immobilization, Microchannel surfaces, Microchips, Protein patterning, Protein-repelling, Specific capture, UV, Antibodies
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Other Biological Topics
Identifiers
URN: urn:nbn:se:kth:diva-164546Scopus ID: 2-s2.0-84901796707ISBN: 9780979806421 (print)OAI: oai:DiVA.org:kth-164546DiVA: diva2:806092
Conference
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, 1 November 2009 through 5 November 2009, Jeju, Japan
Note

QC 20150417

Available from: 2015-04-17 Created: 2015-04-17 Last updated: 2015-04-17Bibliographically approved

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Renberg, Björn
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