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A microwell chip for parallel culture and analysis of stem cells
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Gene Technology. National Institute of Health, United States .
KTH, School of Biotechnology (BIO), Gene Technology.
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2009 (English)In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2009, 1309-1311 p.Conference paper, Published paper (Refereed)
Abstract [en]

With recent findings on the role of reprogramming factors on stem cells, in vitro screening assays for studying (de)-differentiation is of great interest. We developed a miniaturized stem cell screening chip that is easily accessible and provides means of rapidly studying thousands of individual stem/progenitor cell samples, using low reagent volumes. Results presented here include weeklong culturing and differentiation assays of mouse embryonic stem cells, mouse adult neural stem cells, and human embryonic stem cells. The possibility to maintain the cells as stem/progenitor cells over time was shown, as was isolation and clonal analysis.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2009. 1309-1311 p.
Keyword [en]
Clone cells, Cloning, Differentiation (calculus), Mammals, Microchannels, Screening, Cell screening, Human embryonic stem cells, In-vitro, Micro wells, Mouse embryonic stem cells, Neural stem cell, Screening assays, Stem cells
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-164568Scopus ID: 2-s2.0-84901773950ISBN: 9780979806421 (print)OAI: oai:DiVA.org:kth-164568DiVA: diva2:806270
Conference
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, 1 November 2009 through 5 November 2009, Jeju, South Korea
Note

QC 20150420

Available from: 2015-04-20 Created: 2015-04-17 Last updated: 2015-04-20Bibliographically approved

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Lindström, SaraVazin, TandisSandberg, JuliaLundeberg, JoakimAndersson-Svahn, Helene
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
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  • de-DE
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  • nn-NB
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  • Other locale
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Output format
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