Novel bioassay system for single cells
2009 (English)In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2009, 1171-1173 p.Conference paper (Refereed)
In this paper, we propose a novel bioassay system consisting of an active microfluidic device integrated on a microwell plate for high throughput single-cell analysis. Firstly, a CFD (computational fluid dynamics) simulation is performed to determine flow fields in order to assess how fluid flow reaches cells cultured in the wells in the microwell plate. It is then confirmed that the cells are appropriately stained with Calcein AM by fluid flow generated by magnetically driven micropumps. The proposed bioassay system would be a powerful platform for single-cell characterization in a high throughput manner.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2009. 1171-1173 p.
Biophysics, Cells, Computational fluid dynamics, Cytology, Flow of fluids, Microchannels, Microfluidics, Pumps, Throughput, Calcein, CFD (computational fluid dynamics), High throughput, Micro pump, Micro-fluidic devices, Microwell plates, Single cells, Singlecell analysis, Bioassay
IdentifiersURN: urn:nbn:se:kth:diva-164555ScopusID: 2-s2.0-84901772016ISBN: 9780979806421OAI: oai:DiVA.org:kth-164555DiVA: diva2:806311
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, 1 November 2009 through 5 November 2009, Jeju, South Korea
QC 201504202015-04-202015-04-172015-04-20Bibliographically approved