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Improved sensitivity on an allergen lateral flow microarray by means of dendritic amplification
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
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2009 (English)In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2009, 1136-1137 p.Conference paper, Published paper (Refereed)
Abstract [en]

Recently, paper-based substrates have been proposed as an alternative to commonly used activated glass slides for microarray patterning[1], used in conjunction with capillary driven lateral flow of sample and detection reagents through the membrane. While fluorescent detection reagents may be employed to achieve high sensitivity, gold nanoparticles can also be used to allow readout by means of common table top scanners or digital cameras. Here, we demonstrate first results from employing a dendritic, or layer-by-layer, amplification approach for a high-density lateral flow allergen protein microarray, indicating that substantially increased sensitivity can be achieved with very modest increase of assay handling and time requirements.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2009. 1136-1137 p.
Keyword [en]
Allergies, Gold, Detection reagents, Fluorescent detection, Gold Nanoparticles, High sensitivity, Lateral Flow, Nanobeads, Protein microarray, Time requirements, Allergens
National Category
Biological Sciences Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-164536Scopus ID: 2-s2.0-84901752347ISBN: 9780979806421 (print)OAI: oai:DiVA.org:kth-164536DiVA: diva2:806511
Conference
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, South Korea, 1 November 2009 through 5 November 2009
Note

QC 20150420

Available from: 2015-04-20 Created: 2015-04-17 Last updated: 2015-04-20Bibliographically approved

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Gantelius, JesperAndersson-Svahn, Helene
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