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Towards single molecule detection in minute volumes - UV-grafted dna probes with padlock probe detection and RCA amplification
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
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2009 (English)In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2009, 1554-1556 p.Conference paper, Published paper (Refereed)
Abstract [en]

The technique of Immobilizing DNA in extended nano-space (10-1000 nm) was developed. This technique can be used for rolling circle amplification (RCA) and will finally make single DNA detection and nano-immunoassay possible. Here, we combine UV-mediated immobilization to DNA, examine two different methods for immobilization, and couple to padlock probe and RCA. Detection of fluorescence labeled DNA hybridization to RCA demonstrated that DNA analysis in minute volumes succeeded. Our technique can be served to detect single DNA, and we are developing this technique in extended nano-sapce to define the mechnisms of DNA amplification by record RCA in real time.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2009. 1554-1556 p.
Keyword [en]
Locks (fasteners), DNA amplification, DNA detection, DNA hybridization, DNA immobilization, RCA, Rolling circle amplifications, Single-molecule detection, UV-mediated, DNA
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-164549Scopus ID: 2-s2.0-84901770971ISBN: 9780979806421 (print)OAI: oai:DiVA.org:kth-164549DiVA: diva2:806719
Conference
13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, 1 November 2009 through 5 November 2009, Jeju, South Korea
Note

QC 20150421

Available from: 2015-04-21 Created: 2015-04-17 Last updated: 2015-04-21Bibliographically approved

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