Development of a microfluidic concentration gradient generator on a microwell slide for high-throughput cell analysis
2012 (English)In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society , 2012, 1573-1575 p.Conference paper (Refereed)
The present study reports a newly developed, microfluidic concentration gradient generator that adds substantial utility to the high-throughput nature of the microwell slide that we have previously established [1, 2]. Using microfluidic dynamics, we have designed microchannels to deliver a reagent and its dilutant with precisely controlled flow volumes, generating 8 discrete steps of reagent concentration in designated microwells. As proof of concept FITC-labeled dextran and dextran-free water was used to visualize the concentration gradient. The next step will be to perform cancer drug concentration gradient experiments on an animal cell line.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2012. 1573-1575 p.
Cell analysis, High-throughput, Microfluidic concentration gradient, Microwells
IdentifiersURN: urn:nbn:se:kth:diva-165128ScopusID: 2-s2.0-84901752140ISBN: 9780979806452OAI: oai:DiVA.org:kth-165128DiVA: diva2:807638
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 28 October 2012 through 1 November 2012, Okinawa, Japan
FunderSwedish Foundation for Strategic Research
QC 201504242015-04-242015-04-232015-04-24Bibliographically approved