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Perfusion Processes
KTH, School of Biotechnology (BIO), Industrial Biotechnology. (Cell Technology)ORCID iD: 0000-0002-5370-4621
2015 (English)In: Animal Cell Culture / [ed] Mohamed Al-Rubeai, Switzerland Springer: Springer , 2015, 407-443 p.Chapter in book (Refereed)
Abstract [en]

The interest for perfusion is increasing nowadays. This new focus has emerged from a synergy of a demand for disposable equipment and the availability of robust cell separation device, as well as the need for higher flexibility and lower investment cost. The cell separation devices mostly used today are based on filtration, i.e. alternating flow filtration, tangential flow filtration, spin-filter, or acceleration/gravity, i.e. inclined settler, centrifuge, acoustic settler. This paper gives an introduction to the basic concepts of perfusion and its practical implementation. It reviews the actual cell separation devices and describes the approaches used in the field to develop and optimize the perfusion processes.

Place, publisher, year, edition, pages
Switzerland Springer: Springer , 2015. 407-443 p.
Series
Cell Engineering, ISSN 1389-6946
Keyword [en]
perfusion, CHO, Chinese Hamster Ovary cells, mammalian cells, ATF, TFF, centritech, acoustic settler, inclined settler, perfusion rate
National Category
Bioprocess Technology
Research subject
Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-166112DOI: 10.1007/978-3-319-10320-4_13ISBN: 978-3-319-10320-4 (print)OAI: oai:DiVA.org:kth-166112DiVA: diva2:809286
Note

QC 20150508

Available from: 2015-05-01 Created: 2015-05-01 Last updated: 2015-05-08Bibliographically approved

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Chotteau, Veronique

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