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Extended signal peptides in autotransporters are associated with large passenger proteins
KTH, School of Biotechnology (BIO), Industrial Biotechnology.ORCID iD: 0000-0002-3314-6060
KTH, School of Biotechnology (BIO), Industrial Biotechnology.
KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Industrial Biotechnology.ORCID iD: 0000-0002-6979-0069
(English)Manuscript (preprint) (Other academic)
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-166389OAI: oai:DiVA.org:kth-166389DiVA: diva2:810709
Note

QS 2015

Available from: 2015-05-08 Created: 2015-05-08 Last updated: 2015-05-08Bibliographically approved
In thesis
1. Strategies for improved Escherichia coli bioprocessing performance
Open this publication in new window or tab >>Strategies for improved Escherichia coli bioprocessing performance
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Escherichia coli has a proven track record for successful production of anything from small molecules like organic acids to large therapeutic proteins, and has thus important applications in both R&D and commercial production. The versatility of this organism in combination with the accumulated knowledge of its genome, metabolism and physiology, has allowed for development of specialty strains capable of performing very specific tasks, opening up opportunities within new areas. The work of this thesis has been devoted to alter membrane transport proteins and the regulation of these, in order for E. coli to find further application within two such important areas.

The first area was vaccine development, where it was investigated if E. coli could be a natural vehicle for live vaccine production. The hypothesis was that the introduction and manipulation of a protein surface translocation system from pathogenic E. coli would result in stable expression levels of Salmonella subunit antigens on the surface of laboratory E. coli. While different antigen combinations were successfully expressed on the surface of E. coli, larger proteins were affected by proteolysis, which manipulation of cultivation conditions could reduce, but not eliminate completely. The surface expressed antigens were further capable of inducing proinflammatory responses in epithelial cells.

The second area was biorefining. By altering the regulation of sugar assimilation, it was hypothesized that simultaneous uptake of the sugars present in lignocellulose hydrolyzates could be achieved, thereby improving the yield and productivity of important bio-based chemicals. The dual-layered catabolite repression was identified and successfully removed in the engineered E. coli, and the compound (R)-3-hydroxybutyric acid was produced from simultaneous assimilation of glucose, xylose and arabinose.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2015. 104 p.
Series
TRITA-BIO-Report, ISSN 1654-2312 ; 2015:9
Keyword
E. coli, Salmonella, surface expression, autotransport, AIDA-I, lignocellulose, glucose, xylose, arabinose, simultaneous uptake, 3HB
National Category
Biological Sciences
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-166387 (URN)978-91-7595-523-0 (ISBN)
Public defence
2015-06-05, FB52, AlbaNova universitetscentrum, Roslagstullsbacken 21, KTH, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

QC 20150508

Available from: 2015-05-08 Created: 2015-05-08 Last updated: 2015-05-08Bibliographically approved

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Gustavsson, MartinLarsson, Gen

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