DNA melting curve analysis on semi-transparent thin film microheater on flexible lab-on-foil substrate
2012 (English)In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 2012, 797-799 p.Conference paper (Refereed)
This paper presents genotyping on a novel microheater concept using semi-transparent copper microheaters manufactured by roll-to-roll and lift-off on polyethylene napthalate (PEN) foil. Using a mesh structure, heater surfaces have been realized in one single metallization step with a manufacturing robustness higher than conventional meander structures. The thermal distribution of the meshes, evaluated using thermochromic-liquid-crystals (TLC), produced more homogenous heating characteristics compared to meanders. Parylene coated heaters were functionalized using copolymer poly(DMA-NAS-MAPS) to enable covalent DNA immobilization and successful melting curve analysis was performed differentiating between match and mismatch oligonucleotides.
Place, publisher, year, edition, pages
2012. 797-799 p.
Heating equipment, Melting, Oligonucleotides, DNA immobilization, Functionalized, Meander structures, Melting curve analysis, Mesh structures, Polyethylene napthalate, Semi-transparent, Thermal distributions, Microelectromechanical devices
IdentifiersURN: urn:nbn:se:kth:diva-167651ScopusID: 2-s2.0-84901784864ISBN: 9780979806452OAI: oai:DiVA.org:kth-167651DiVA: diva2:816854
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 28 October-1 November 2012, Okinawa, Japan
QC 201506042015-06-042015-05-222015-06-04Bibliographically approved