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DNA melting curve analysis on semi-transparent thin film microheater on flexible lab-on-foil substrate
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2012 (English)In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 2012, p. 797-799Conference paper, Published paper (Refereed)
Abstract [en]

This paper presents genotyping on a novel microheater concept using semi-transparent copper microheaters manufactured by roll-to-roll and lift-off on polyethylene napthalate (PEN) foil. Using a mesh structure, heater surfaces have been realized in one single metallization step with a manufacturing robustness higher than conventional meander structures. The thermal distribution of the meshes, evaluated using thermochromic-liquid-crystals (TLC), produced more homogenous heating characteristics compared to meanders. Parylene coated heaters were functionalized using copolymer poly(DMA-NAS-MAPS) to enable covalent DNA immobilization and successful melting curve analysis was performed differentiating between match and mismatch oligonucleotides.

Place, publisher, year, edition, pages
2012. p. 797-799
Keywords [en]
Heating equipment, Melting, Oligonucleotides, DNA immobilization, Functionalized, Meander structures, Melting curve analysis, Mesh structures, Polyethylene napthalate, Semi-transparent, Thermal distributions, Microelectromechanical devices
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:kth:diva-167651Scopus ID: 2-s2.0-84901784864ISBN: 9780979806452 (print)OAI: oai:DiVA.org:kth-167651DiVA, id: diva2:816854
Conference
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, 28 October-1 November 2012, Okinawa, Japan
Note

QC 20150604

Available from: 2015-06-04 Created: 2015-05-22 Last updated: 2015-06-04Bibliographically approved

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