RhoD is a Golgi component with a role in anterograde protein transport from the ER to the plasma membrane
2015 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 333, no 2, 208-219 p.Article in journal (Refereed) Published
RhoD is a member of the Rho GTPase family and it coordinates actin dynamics and membrane trafficking. Activation of RhoD results in formation of filopodia, dissolution of stress fibers, and the subsequent formation of short actin bundles. In addition, RhoD localizes to early endosomes and recycling endosomes, and has a regulatory role in endosome trafficking. In this study, we report on a function of RhoD in the regulation of Golgi homeostasis. We show that manipulation of protein and activation levels of RhoD, as well as of its binding partner WHAMM, result in derailed localization of Golgi stacks. Moreover, vesicle trafficking from the endoplasmic reticulum to the plasma membrane via the Golgi apparatus measured by the VSV-G protein is severely hampered by manipulation of RhoD or WHAMM. In summary, our studies demonstrate a novel role for this member of the Rho GTPases in the regulation of Golgi function.
Place, publisher, year, edition, pages
2015. Vol. 333, no 2, 208-219 p.
Golgi, Rho GTPases, RhoD, VSV-G, WHAMM
IdentifiersURN: urn:nbn:se:kth:diva-166900DOI: 10.1016/j.yexcr.2015.02.023ISI: 000353666100009PubMedID: 25746724ScopusID: 2-s2.0-84927911493OAI: oai:DiVA.org:kth-166900DiVA: diva2:818791
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Cancer Society, 110549Swedish Research Council, K201066P20582044The Karolinska Institutet's Research Foundation
QC 201506092015-06-092015-05-212015-06-12Bibliographically approved