Parallel cDNA synthesis from thousands of individually encapsulated cancer cells: Towards large scale single cell gene expression analysis
2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1737-1739 p.Conference paper (Refereed)
We present microfluidic droplet-based cDNA synthesis of 65 000 individually isolated lung cancer cells in parallel. Cells are encapsulated, individually lysed and the RNA from each cell is reverse transcribed in droplets at a massively parallel scale resulting in thousands of droplets each containing the cells gene expression profile encoded in stable DNA for downstream analysis (figure 1). This could be used for distinguishing between different cell types and study heterogeneity within a cell sample at high throughput scale.
Place, publisher, year, edition, pages
2013. Vol. 3, 1737-1739 p.
CDNA synthesis, Droplet microfluidics, High throughput, Single cell analysis
Other Biological Topics
IdentifiersURN: urn:nbn:se:kth:diva-168809ScopusID: 2-s2.0-84907352100OAI: oai:DiVA.org:kth-168809DiVA: diva2:819214
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany
QC 201506102015-06-102015-06-092015-06-10Bibliographically approved